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Importantly, an additional experiment showed that LASSS-treated BSA (after the removal of un-reacted LASSS) exhibited nitration resistance to the optimal peroxynitrite-treatment (1:2000 ratio), as shown in supplementary Figure 2, suggesting a model that LASSS-induced chemical modification mechanism exerts a nitration-inhibitory effect on a wide range of proteins susceptible to tyrosine nitration

The oocytes were analyzed using fluorescence microscopy (Eclipse 80i, Nikon Instruments Europe, Amsterdam, The Netherlands) at 20 magnification with an Ex filter of 475/30 nm, a DM 405 nm, and an Em filter of 530/40 nm

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