Osman is a co-author and an Associate Editor of the journal
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Quantitative PCR was performed in technical triplicate using 20 ng cDNA, 1 Power SYBR Green PCR Master Mix (Thermo Fisher Scientific) and 0.5 M forward and reverse primers (Supplementary Table 1), using an ABI 7900HT Real-Time PCR System (Applied Biosystems)
By inference from related viruses, RNA synthesis is likely to be semiconservative and asymmetric: the positive-strand genome serves as a template to make a negative-strand intermediate
Although enriched in proteins with higher levels of total conservation, there are examples of conserved immunogenic epitopes in most, if not all, viral proteins [43, 99, 100]