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USP-NF: United States Pharmacopeia and National Formulary

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Conjugated bilirubin reacts quickly through the Van den Bergh reaction (VdB) due to the accessibility of its central methane group while non-conjugated bilirubin reacts very slowly to Diazo reactive substances because of its internalized hydrogen and the methane bridge which is inaccessible to the reactive substance (13, 21)