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Cryo-EM grid preparation For grid preparation, 3 uL of 3 mg/mL purified OCTN2 was applied to a freshly glow discharged holey gold grid, with UltraAuFoil R0.6/1, 300 mesh used for inward-facing, and UltrAuFoil R1.2/1.3 300 mesh grids used for OCTN2 occluded and OCTN2 ipratropium

(6) did not have inpatient medical care, ER or hospitalization