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The plate containing the frozen cells were lyzed with Pierce RIPA Lysis and Extraction Buffer (Thermo Fischer Scientific) supplemented with 1 cOmplete Protease Inhibitor Cocktail (Roche) (220 L/well) for 10 min on ice on a tilting board

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Hoff G, Bernklev T, Johnsen L, Reitsma L, Sina D, Lauzike A, Gibbs C, Carlsen E, Lende TH, Narvestad JK, Kildahl R, Omdal R, Kvaly JT, Siland H