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### Step 4: Draw the Bacteriostatic Water Insert the needle of your 3mL syringe through the BAC water vial stopper

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Recombinant proteins in the supernatant were purified using nickelSepharose resin (GE Healthcare) and initially washed with wash buffer B1 (50 mM Tris, 500 mM NaCl and 30 mM imidazole, pH 8.0) until no protein was detected in the eluent using the Bradford reagent

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