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mobile phase A: acetonitrile: water = 6:4 (v/v, containing 10 mM ammonium acetate)

After washing in blocking buffer diluted 1:1 with PBS and 3 washes in PBS, retinas were incubated for 1 h at RT with secondary antibodies diluted in blocking buffer

This exposed the proximal course of the left circumflex coronary artery (LCx) just distal to its bifurcation from the left main coronary artery

6 Lee A, Cardel M, Donahoo WT

RT-qPCR was performed using the TB Green Premix Ex Taq Kit on an Applied Biosystems QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher Scientific, Waltham, MA, USA) per the following conditions: Denaturation at 95 C for 30 s