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This conventional method, for some long peptides and peptide fragments with strong hydrophobicity, is easy to form beta-sheet secondary structure through intramolecular hydrogen bond during synthesis, thereby causing shrinkage of resin and resulting in coupling difficulty

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52 Following 24-h incubation, the cells were transferred to Costa microtiter plates (150,000 cells/well, PerkinElmer) to achieve a 5%10% specific binding