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Patients on metformin HCl at a dosage of at least 1,500 mg/day were randomized following a 2-week placebo lead-in period to glipizide or dapagliflozin (5 mg or 2.5 mg, respectively) and were uptitrated over 18 weeks to optimal glycemic effect (FPG 300 mg/g)

This is the pathway semaglutide completely misses

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For ultrafiltration experiments, 0.5 ml Amicon Ultra centrifugal filters with 10 kDa cut-off were used (Merck Millipore Ltd, Ireland)