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We generated UBE2m R169K mutants and compared their methylation levels with that of the wild-type UBE2m using immunoprecipitation to validate this finding

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However, basal somatostatin secretion and responses to glucagon were low at 3.5 mmol/l glucose (reflecting only weak glucose-stimulated somatostatin secretion), and we therefore focused on investigating somatostatin at higher glucose concentrations

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