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While primers sequence for IL8, HSP27 and SOD1 were designed using Primer3 ( and Primer-BLAST ( Primer specificity was verified by running PCR for 40 cycles at 95 C for 10 seconds and 60 C for 60 seconds, followed by gel electrophoresis on a 3% agarose gel stained with SYBR safe DNA gel stain

Therefore, based on state-of-the-art evidence from PE literature (redox system, mitochondrial function/structure, and immunology), we will propose PE as a potential therapeutic strategy on vitiligo disease as summarized in Table 1

Dietary data were converted into categorical variables, using the 50th percentile of the sample size as the cut-off point to classify them into low and high groups