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Culture was scaled up into a 1 L flask and grown for a further 4 hours, and protein production induced with 0.5 mM IPTG for 4 hours

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The possible allosteric effect of reactive oxygen species on GPx enzyme can lead to an increased activity through increased lipid peroxidation ( Reference Levin, Cogan and Levy 40 )

The MOTS-c analog CB4211 was well-tolerated in a Phase 1 trial with 85 participants and no serious adverse events