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Synergistic effects of snail and quercetin on renal cell carcinoma Caki-2 by altering AKT/mTOR/ERK1/2 signaling pathways

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Lysis was achieved through five to six iterative freeze-thaw cycles: frozen samples were thawed at 23 C in a thermomixer (Thermomixer Comfort, Eppendorf) until 60-80% liquefied, then held on ice until fully thawed, before being rapidly refrozen in liquid nitrogen