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Western blotting Frozen cortex or hippocampal samples were homogenized in 50 mM TrisHCl (pH 7.5), 150 mM NaCl containing 1% Triton X-100, protease inhibitor cocktail and phosphatase inhibitor cocktail

To investigate what underlines changes in Ca 2+ transients, we assessed levels and phosphorylation state of three major proteins that control the time constant and amplitude of Ca 2+ transient: Na + Ca 2+ exchanger (NCX1), sarco-endoplasmic reticulum Ca 2+ -ATPase (SERCa2), and phospholamban (PLN) (Figure 5C)

The MTHFR C677T and DeltaDNMT3B C-149T polymorphisms confer different risks for right- and left-sided colorectal cancer