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With this in mind, we adapted an alternative approach to hydrophobize the surface of GSH-AuNCs that relies on the electrostatic interaction between hydrophobic cationic phase transfer agent (namely dodecylamine, DDA) and the negatively charged GSH-AuNCs

These studies have generally demonstrated positive therapeutic outcomes, but translation to clinical applications requires careful consideration of species differences and experimental limitations

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So, for example, during expression and purification of a recombinant protein, it may have a disulfide bond form, which wouldnt normally form in the proteins natural context