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As an alternative approach to improve in vivo stability of the fusion molecule, we then engineered a disulphide bridge into the exendin-4 peptide moiety (Ab#2_DSB#1) as this has been shown to improve in vitro and in vivo stability of both GLP-1 and exendin-4 analogue peptides 36

The dilution ratio and manufacturer of antibodies which used are shown in Table 1

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[DOI] [PMC free article] [PubMed] [Google Scholar] 36.Ilmarinen P., James A., Moilanen E., Pulkkinen V., Daham K., Saarelainen S., Laitinen T., Dahln S.E., Kere J., Dahln B., et al