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By targeting SLC33A1 to manipulate glutathione export and accumulation within the ER, it may be possible to induce toxic oxidative imbalances selectively in cancer cells, thus offering a novel strategy for therapeutic intervention
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Materials and methods Cell culture Human acute monocytic leukemia cells, THP1, and the mouse leukemic monocyte cell line, RAW264.7, were cultured using RPMI-1640 medium modified to exclude calcium nitrate and supplemented with 2.05 mM L-Glutamine (Gibco, C11875500BT), 10% fetal bovine serum (FBS) (ExCell, FSP500), and a 1% penicillinstreptomycin mixed solution (Abiowell, AWH0529a)
doi: 10.1007/s12325-024-02919-5