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The qRT-PCR reaction was made up of 4.5 L diluted (20X) cDNA template, 0.25 L forward and 0.25 L reverse primers of GSTs, and 5 L of Maxima SYBR Green qPCR Master Mix (2X) (Thermo Scientific, Waltham, MA, USA) in double-distilled water at a final volume of 10 L

Invest Ophthalmol Vis Sci 54:34003409 Davison K, Cote S, Mader S, Miller WH (2003) Glutathione depletion overcomes resistance to arsenic trioxide in arsenic-resistant cell lines

Reconstitution is the process of adding a liquid, typically bacteriostatic water, to a freeze-dried (lyophilized) powder to turn it into a sterile liquid solution suitable for research administration

Peter Attia MD

In: OHehir RE, Holgate ST, Sheikh ABT-MAE (eds)