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For the best performance, typical imaging time should be 6 cells were analysed for each imaging experiment, and all the imaging experiments were repeated at least twice with one set of representative data shown in each figure

Standard is weekly same-day dosing, but timing within that day matters significantly

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Biological analyses were performed on stool samples (16S rRNA sequencing) and blood serum (ELISA for oxytocin, inflammatory and neuroinjury markers), while behavioral outcomes were assessed using SRS, ABC and CGI