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The maximal velocity (Vmax) and the Michaelis constant (K m ) of the tyrosinase activity were determined by the Line weaver Burk plot at various concentrations of L-DOPA (0.25, 0.5, 0.75 and 1 mM) as a substrate

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Changing the level of ROS or antioxidants to disrupt this balance will lead to T cell hyperresponsiveness or hyporeactivity, which may lead to the development of various pathology (Gelderman et al., 2007)

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