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For most of these types of lipidation, alkyne- or azide-modified lipid precursors have been generated that enable metabolic labeling of lipidated proteins and, following CuAAC tagging and then profiling by in-gel fluorescence or affinity enrichment for identification by MS-based proteomics (Figure 66)

Proc Natl Acad 1987

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Unlike complex biological extracts, synthetic peptides provide clearly defined structures, allowing researchers to isolate and analyse specific biochemical responses

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