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canettii C59 strain containing pJV53H in order to obtain metE and metH mutants in this strain following the same procedure explained above for M
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Immunocytochemistry analysis was performed with an anti-GFP primary antibody (rabbit polyclonal anti-GFP, 1:1000, Thermo Fisher Scientific Inc., Waltham, MA, USA) and a goat anti-rabbit IgG (H + L) secondary antibody (1:400, Alexa Fluor 488, Invitrogen - Thermo Fisher Scientific Inc., Waltham, MA, USA)
