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Isopropyl -D-1-thiogalaytopyranoside (IPTG) was added at a concentration of 0.5 mM for overnight induction at 20C

The plasmid system is relatively stable but has low cutting and editing efficiency, and continuous expression of Cas9 may increase off-target effects

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Reduced glucose, insulin and IGF-1 levels ormore generallyDR activate an energy-sensing network consisting of AMPK, SIRT1, PPAR and PGC-1 with the potential to counteract tumor cell proliferation