Yes, thigh injections are an approved administration site for GLP-1 receptor agonists like semaglutide and tirzepatide, alongside abdomen and upper arm options
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To determine whether the colistin-induced depolarization was the result of the opening of the mitochondrial permeability transition pore (mPTP), mitochondria were co-incubated with colistin and cyclosporin A, a well-characterized desensitizer of the mPTP 31,32
To compare these samples, the images were merged using gel imaging software, Image Lab 6.0.1 software (Bio-Rad), to align the protein markers (from colorimetric scans) for molecular weight alignments before excising the gel regions for MS analyses and peptide sequencing to identify the proteins they contained