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Binding studies employed tritiated small molecule HGF analogs to map the distribution of HGF/c-Met receptors in the brain, establishing a correlation with known AT4 receptor densities

To address the critical issue of BBB permeability, studies using [H]Dihexa and [C]inulin in rats revealed that Dihexa avidly concentrated in all examined brain regions including the prefrontal cortex, hippocampus, hypothalamus, striatum, thalamus, midbrain, brain stem, and cerebellum, compared to blood

30 ug of the protein was the separated using 10% SDS-PAGE and electroblotting onto polyvinylidene difluoride (PVDF) membranes

This research explores a novel therapeutic avenue by targeting the brains renin-angiotensin system (RAS) and its interaction with the hepatocyte growth factor (HGF)/c-Met receptor system [2]