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NMR spectroscopy NMR experiments were performed using a Bruker 900 MHz NMR spectrometer equipped with a cryogenic probe (KBSI, Ochang) and a Bruker 600 MHz NMR spectrometer equipped with a cryogenic probe (GIST, Gwangju)

Notably, animal models have demonstrated that transferring dysbiotic microbiota from CFS/ME patients to germ-free mice can induce fatigue-like behaviors, supporting a causal role for the microbiome in disease pathogenesis ( 3.5.4 Gut microbiota-based therapeutic interventions 3.5.4.1 Probiotics Given these findings, probiotic supplementation, particularly with strains of Lactobacillus and Bifidobacterium , has shown potential to restore microbial balance, enhance SCFA production, and reduce inflammation in CFS/ME patients (Carrasco-Querol et al., 2024

e ., protection of stress ulcers)

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MicroRNAs in mucosal inflammation