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Fmoc-protected amino acids were coupled to the resin using Oxyma Pure (0.25 M) and N, N-diisopropyl carbodiimide (0.125 M) as the activator and activator base, respectively

Haematoxylineosin staining Haematoxylineosin staining was used to investigate the morphologic and pathologic alterations of the cardiac tissue

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Prepared cDNA was used in the qPCR analyzer (StepOne, Applied Biosystems, Singapore) using MAXIMA SYBR Green qPCR Master Mix with the following program: 1 cycle at 95 C for 10 min

Nearly identical assertions are also being pursued against other GLP-1 receptor agonists, such as Wegovy, Mounjaro, Rybelsus, Saxenda, and Trulicity