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It is recommended to use an ice compress on the affected area for intervals of 10 to 15 minutes

Protein bands were visualized using Immobilon Western Chemiluminescent HRP Substrate (Merck Millipore, Billerica, MA, USA) and a ChemiDoc MP Imaging System with Image Lab Touch Software Version 1.0 (Bio-Rad Laboratories, Hercules, CA, USA)

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