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Cerebrospinal fluid samples were thawed then 150 L immediately mixed with 150 L of methanol/water 50/50, thoroughly mixed on a vortex mixer, incubated in an ultrasonic bath for 5 min and incubated on ice for 20 min

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She required multiple treatments with C1-INH and icatibant acetate, resulting in symptomatic improvement

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References Clinical Pharmacology powered by Elsevier (ClinicalKey) Tirzepatide Monograph