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Muscle, in particular, requires IRS-1 for IGF-I mediated hypertrophy, because a transgenic cross of IRS-1 +/ mice with a mouse line overexpressing IGF- I blocked the anticipated increase in skeletal muscle mass, even though IGF-I could rescue the growth deficits in other tissues (brain, intestine, and heart) 55
In addition, in the New Zealand obese (NZO) mice, in contrast to GLP-1, the GLP-1 and E2 conjugate significantly decreased food intake and prevented obesity, thus preventing T2D 17
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Fos-positive neurons in three specimens from each animal were counted using ImageJ
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