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Studies have shown that AOD9604 acts through a combination of pathways, including beta-3 adrenergic receptor upregulation, cAMP-PKA-HSL signaling pathway activation, and acetyl-CoA carboxylase inhibition

Thiyl radicals are readily formed through homolytic cleavage of the sulfhydryl S-H bond due to low bond dissociation energies of around 87 kcal mol 1 (Dns et al., 1 or the water-soluble 2,2'-azobis[2-(2-imidazolin-2-yl)propane]-dihydrochloride (VA044) 2 radical initiator (Figure 1)

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complete GS-like gene sequences are available under Dryad accession doi:10.5061/dryad.7vd0160)