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After bombardment, onion peels were incubated for 2647 h on B5 medium ( PHYLOGENETIC ANALYSIS Phylogenetic analysis was performed using MEGA version 6 software (Tamura et al., 2013) based on the ClustalW multiple alignment

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Animals were maintained in a 15:9 h photoperiod of daily light/dark cycles to match the natural photoperiod at the collection sites (metal halide lights, with levels maintained at 150250 mol m 2 s 1 ) and in dimmed light conditions to mimic their natural sciaphilous environment (the tanks were under a canopy of 70% light-reducing shade-cloth)

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Physical chemistry: principles and applications in biological sciences