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cAMP assay OA (10 M) was added to 0.5 ml of cell suspension (10 6 cells/ml) in the presence of 3-isobutyl-1-methylxanthine (IBMX, 10 M), and the reaction was terminated after 60 s with 6% cold trichloroacetic acid (Vol/Vol)

doi: 10.1074/jbc.274.36.25481 45 JhangK

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Clinically, melatonin has been reported to reduce the risk of hemorrhagic transformation after tissue plasminogen activator (t-PA) therapy in ischemic stroke (150, 151), suggesting a potential role in improving thrombolytic safety through modulation of BBB permeability