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Recombinant TGR was subsequently expressed in the Escherichia coli strain BL21(DE3) in the presence of pSUABC [14] in LB medium supplemented with 20 M flavin adenine dinucleotide, and otherwise conditions for optimal selenoprotein expression were followed as described [15]

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Iron malabsorption can also be due to gastrointestinal disorders of autoimmune etiology (celiac disease, atrophic gastritis, IBD), which are often associated with autoimmune thyroid diseases [59, 60]