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The messenger RNA expression levels of the (E) AMPK, (F) SIRT6, (G) SREBP-2, (H) HMGCR, (I) SQLE, (J) LXR, (K) CYP7A1, (L) LDLR, and (M) PCSK9 genes were analyzed using quantitative RT-PCR and normalized to that of the ACTB gene