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pH is measured with calibrated electrochemical pH meters per USP and validated at each stability time point

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Subsequent introduction of two random mutations, Y444A and G484A, to D433N EcGGT variant further enhanced catalytic efficiency for GL-7-ACA substrate by 50-fold (Yamada et al., 2008)

This work was funded by the METROFOOD-CZ Research Infrastructure Project (MEYS grant number: LM2018100 and LM2023064) and internal grant of the UCT Prague No