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After solubilization, the samples were analyzed for H and C content using dual window scintillation counting
In organotypic hippocampal slice cultures, Dihexa and Nle-AngIV similarly augmented spinogenesis, with control slices showing 7 spines per 50-m dendrite length, compared to 11 for both Dihexa and Nle-AngIV treated slices [1]
Electrophysiological recordings further supported this, revealing that Dihexa caused a 1.6-fold increase and Nle-AngIV caused a 1.7-fold increase in the frequency of AMPA-mediated mEPSCs compared to vehicle-treated neurons, indicating an expansion of functional synapses without altering individual synapse properties such as amplitude, rise, or decay times
Binding studies employed tritiated small molecule HGF analogs to map the distribution of HGF/c-Met receptors in the brain, establishing a correlation with known AT4 receptor densities
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