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The third group (n = 14) was fed the control diet for one week, followed by the BSO diet for the remaining study period

EGF (Epidermal Growth Factor) and sh-Oligopeptide-1: the keratinocyte signal Epidermal growth factor was identified in 1962 by Stanley Cohen at Vanderbilt, work that earned the Nobel Prize in 1986

PaSTRy Solutions of EV-F3 and additive at either 100 or 1000 molar excesses (corresponding to 0.1 mM or 1 mM final concentration) were aliquoted into a 96-well PCR plate containing SYTO9 dye (Thermo Fisher Scientific) in triplicate with 50 mM HEPES, pH 8.0, 200 mM NaCl buffer, before centrifugation for 3 min at 4 C