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Materials and methods Cell lines and chemicals Primary and LN-met melanoma cell lines WM35, WM75, WM1552, WM1862C, WM115, WM266-4, WM239A, and WM165-1 were cultured in tumor specialized medium (TSM) [10] with 2% heat-inactivated FBS and 1% penicillin and streptomycin (pen+strep)

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