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### Step 6: Let It Dissolve Set the vial down and wait 1-2 minutes

Always consult your healthcare provider before beginning any new wellness routine

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There have also been concerns raised in the scientific literature regarding cardiovascular safety

BAT was chopped in 0.2% (w/v) collagenase type I solution dissolved in DMEM supplemented with penicillin (100 U/mL), streptomycin (100 g/mL), 2 mM glutamine, 10 mM HEPES, and 1.5% BSA, incubated at 37 C for 30 min in a stirring bath and filtered through a 250 m silk filter