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Traditional methods have typically included high performance liquid chromatographic assays and are characterized by underestimation of GSH and overestimation of GSSG due to the autooxidative properties of GSH ( Statements Author contributions EK: Conceptualization, Data curation, Formal Analysis, Investigation, Methodology, Project administration, Resources, Software, Supervision, Validation, Visualization, Writing original draft, Writing review and editing

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Although the overall data were not statistically significant, some items were significant at the 90% level for females