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The ingredients in supplements, including vitamins, minerals, herbs, and other botanicals, can have unintended negative consequences when combined with some medications, other supplements, foods, and alcohol ( For example, vitamin E supplements may increase the risk of bleeding in those on blood-thinning medications, while calcium supplements may limit the effectiveness of antibiotics and reduce the absorption of iron from foods and supplements ( Some supplements may also affect the accuracy of lab results

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Cells were purified by several centrifugations and cultured in DMEM medium supplemented with 10% FBS, HEPES (4.5 mM), penicillin (100 U/mL), L-glutamine (2 mM) and sodium bicarbonate (0.17 M)