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Cell pellets were resuspended in LDOX lysis buffer (20 mM potassium phosphate, 200 mM NaCl, pH 7.4, 0.01 mg ml 1 DNAseI) to an OD 600 of 140 and lysed by two rounds of sonication for 2 min at 50% amplitude with 2 s intervals using a Sonopuls (Bandelin) sonicator

Yes, it is suitable for both vegetarians and vegans

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Agro-wastes and agro-industrial wastes have been utilized by the mushrooms and provide a good source of protein (36, 37)

Then theres IGF-1 LR3, a modified version of a natural protein (IGF-1) linked to muscle growth