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Electronic Ligand Builder and Optimization Workbench (eLBOW): a tool for ligand coordinate and restraint generation

Once the culture has been induced with IPTG, do not allow the cells to grow for extended periods of time, as cell lysis can occur and release proteases into the cytoplasm that could degrade the fusion protein

And you see that with theophylline

The article notes that post-activation allowances vary by formulation, with some preparations having a room-temperature window and others not, and that your pharmacy's label states which one applies to what you were dispensed

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