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Step 2: Monitor your symptoms

Hypothalamic neurogenesis and maturation After 14 days of hypothalamic differentiation, hypothalamic progenitors were dissociated with a mixture of TrypLE, papain (Worthington Biochemical), and DNAse I (Worthington Biochemical) with a subsequent 2 washes in N2B27 medium supplemented with 10 M Rock inhibitor

DOI: Publisher Name: Humana Press Print ISBN: 978-0-89603-521-8 Online ISBN: 978-1-59259-260-9 eBook Packages: Springer Protocols Keywords These keywords were added by machine and not by the authors

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Gonadotropic derangement Gonadotrophin-releasing hormone (GnRH) is secreted in a pulsatile manner from the hypothalamus to the pituitary gland and concentrations of luteinizing hormone and FSH are influenced by circulating oestradiol, progesterone and leptin 125