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GST-pulldowns using 293T cell lysates Pull downs for mass spectrometry : clarified lysates from 450 mL 2xTY cultures containing bacteria expressing recombinant GST, GST-S tails (product of pJC149, pJC150, pJC247) were thawed

Step-wise elutions of 2.5%, 5% and 7.5% elution buffer were performed to remove any cleaved target protein bound tag-independently to the affinity column

Cardiac magnetic resonance imaging can further quantify the proportion of fatty acids in EAT, providing complementary information to CT imaging

It is most commonly used in injections and is retained in the body for longer

The B12 we use is the natural form of B12 known as methylcobalamin