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GDNF and BDNF can also alleviate mitochondrial dysfunction and apoptosis induced by neurotoxins like MPTP and 6-OHDA, highlighting their potential as therapeutic targets (19, 20)

Before biotinstreptavidin pulldown, magnetic streptavidin beads (Thermo Fisher Scientific, 88817) were washed three times in 0.2% SDS in 50 mM HEPES

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As a control, one of the infected precleared lysates was incubated on the beads without the primary antibody (no first antibody (NFA))

We used the melanin index value, which reflects the skin melanin content, as an objective outcome indicator, and we added subjective assessments by the investigator and by study participants