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The conditions, such as a 75 mM sodium phosphate buffer (pH 3.0) with 1.7 mM 1-octanesulfonic acid, 25 M EDTA, 10% methanol, and a flow rate of 1 mL/min, are optimized for precision and sensitivity, ensuring accurate neurotransmitter profiling at a column temperature of 25C

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pyrenoidosa , glycine decreased from 48.67 mg g -1 in the control to 46.13 mg g -1 at 300 g L -1 Pb, then increased markedly to 59.99 mg g -1 at 500 g L -1 Pb, corresponding to an increase of 23.3% relative to the control