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Moreover, peroxidative ROS levels depend on the caution of the antioxidant systems, which shield cells from oxidative harm, incorporate various peroxisomal enzymes, such as catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPX), and peroxiredoxins (PRDX), as well as low molecular weight non-enzymatic antioxidants [17]

Sections were incubated with primary antibodies (PCNA 1:400, caspase-3 1:200, caspase-9 1:250, ALDH-1 1:250, E-cadherin 1:100, vimentin 1:800 and cytokeratin 1:60) and relevant secondary antibodies

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Pathohistological analysis After sacrificing the respective tissue was dissected, fixed in buffered formalin (pH 7.4) for 24 hours and embedded in paraffin using standard procedures