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Growth of additional strains was also tested on diamide (D), including the new sulphonyl urea resistant tps1 strain generated in this study (tps1::ILV1), and tps1 strains expressing Tps1 proteins carrying the point-mutations R22G or Y99V in the G6P binding pocket of the active site (tps1::R22G and tps1::Y99V, respectively)

Liver enzymes spike, inflammation sets in, and in severe cases, the liver can begin to fail within days

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Patients with more severe or longstanding deficiency may take longer to notice the full benefit